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. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Circulation. 2010 Nov 15;122(25):2669–2679. doi: 10.1161/CIRCULATIONAHA.110.982298

Figure 2. CaMKII phosphorylation induces RyR2 mediated SR calcium leak in permeabilized myocytes.

Figure 2

(A) Respresentative confocal line-scan images obtained in permeabilized cardiomyocytes from WT and S2814D mice before and after activation of endogenous CaMKII. (B) Quantification of Ca2+ spark frequency (CaSpF) in cardiomyocytes isolated from WT, S2814D and S2814A mice in the absence (−) or presence (+) of CaMKII and CaMKII inhibitor AIP. (C) SR calcium content (ΔF/F0) in permeabilized myocytes in the absence (−) or presence (+) of CaMKII. (D) Ratio of calcium spark frequency (CaSpF) to SR calcium content. WT myocytes had a significantly increased CaSpF/SR content ratio in the presence of CaMKII. N is indicated in bar graphs. 2-way repeated measures ANOVA was used to compare groups. *P<0.05, **P<0.01, *** P<0.001 vs. same genotype without CaMKII; # P<0.05 vs. WT.