Figure 2.
DSB formation, meiotic progression, and sporulation in a dmc1 mek1-as1 diploid. A dmc1 mek1-as1 diploid (NH520::pLW19) was transferred to sporulation medium at 30°C. Aliquots were taken at the indicated time points and assayed for various meiotic parameters. (A) Meiotic progression was followed by 4,6-diamidino-2-phenylindole staining cells at different time points in the absence of inhibitor. For sporulation, a final concentration of 1 μM 1-Na-PP1 was added at various time points indicated by squares, the cells were returned to 30°C until 48 h when the number of asci were counted by light microscopy. Sporulation is indicated by a dashed line. At least 200 cells were counted for each measurement. (B) DSB formation at the YCR048w hotspot measured in the absence of inhibitor as described in Figure 1.