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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2011 Jan 28;22(2):319–328. doi: 10.1007/s13361-010-0030-7

Figure 2.

Figure 2

(A) Isolation of Syk interacting partners with anti-Syk antibody and by GFP nanotrap and GFP Ab, probed by Western blot using anti-Syk antibody. (B) Isolation of γ-tubulin in the lysate, flowthrough (FT), and elution fractions from Syk-EGFP DT40 B cells using the GFP nanotrap and GFP MAb, and analyzed by Western blotting with anti-γ-tubulin antibody. (C) Western blotting analysis of CrkL in the Syk-EFGP and Syk-deficient lysate and Dips using GFP nanotrap affinity purification with anti-CrkL antibody