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. 2011 Apr 8;88(4):499–507. doi: 10.1016/j.ajhg.2011.03.012

Figure 4.

Figure 4

GDP- and GTP-Binding Properties of Wild-Type and Disease-Associated Mutant Forms of RAB18

(A) Wild-type RAB18 binds GDP similarly to RAB5A and RAB35, whereas RAB18(Arg93del), RAB18(Leu24Gln) and the negative control protein GST do bind GDP.

(B) RAB18 binds to GTP, but RAB18(Arg93del) and RAB18(Leu24Gln) are defective for GTP binding. Error bars indicate the standard deviation from the mean (n = 3). The bead-bound pool of the RABs is shown in the stained immunoblots below the graphs. All are of similar quality and bind to the glutathione beads equally.