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Distribution of detection levels for graded assays. Distributions of the detection levels are plotted for each group of graded assays (A–D). Detection levels (plotted on log2 scale) are estimations of assay sensitivity determined from four-point response curves as part of assay characterization. Calculation of protein concentration assumes enrichment from 10 μl plasma and equal stoichiometry between peptide and protein (i.e. complete trypsin digestion).
