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An example assay characterization report for the peptide YLAPSGPSGTLK from the protein FSCN1. A, Response curves for each of three monitored transitions. B, Example chromatograms for each transition at two concentrations (0.05 fmol/μl and 0.5 fmol/μl heavy peptide; 10 fmol/μl light peptide). Traces show the chromatograms for the (heavy) stable isotope-labeled peptide (in blue) overlaid on traces of the (light) peptide (in pink).
