Figure 2.
GAD67-immunoreactive neurons in FPC of HRM and WTM. (Upper Left) A schematic representation of a coronal section of FPC (0.74 mm anterior to Bregma) illustrating the subdivisions of superficial layers (I–IV) and deep cortical layers (V–VI) of the M2, Cg1, and Cg2 regions of the FPC, and Right is the percentage of GAD67-immunoreactive cells in the HRM, as compared with the WTM. Student's t test comparing HRM with WTM. *, P < 0.01;**, P < 0.001. (Lower) Photomicrographs of 40-μm sections through the FPC of the WTM (Left) and HRM (Right) immunolabeled for GAD67. Note that in the HRM there is a decreased number of GAD67-immunopositive cells in the Cg1 and M2 regions, as well as a decrease in the surrounding neuropil expression of GAD67. Each value represents the mean of eight to nine animals. Standard error range from 2–10% of the mean. (Bar = 300 μm.)