FIG. 4.
IL-21-producing CD4 T cells are more functional in elite controllers. CD45RO+ CD4 (A) and CD8 (B) T cells that produced IL-21 were analyzed for their ability to coproduce IFN-γ, IL-2, or TNF-α using SPICE. The magnitudes of virus-specific mono- and multifunctional CD4 and CD8 T cells producing at least IL-21 from PHI, EC, VC, and P cohorts were assessed. Horizontal bars indicate the median percentages of IL-21+ T cells. Statistical comparisons were made using the two-tailed nonparametric Mann-Whitney U test. **, greater than 3 or 4 functions; P < 0.03 versus PHI, VC, and P cohorts.