Fig. 2.
Diet-induced metabolic dysfunction in TIMP-2 KO mice. A, At the study start, plasma glucose, insulin, and leptin levels were comparable in WT and TIMP-2 KO mice, suggesting that KO mice were not overtly diabetic and TIMP-2 KO hyperphagia was not due to reduced leptin. B, At the study termination, even though chow-fed TIMP-2 KO mice weighed considerably more than WT mice, plasma glucose, insulin, and leptin levels were comparable. C, In contrast, HFD-fed TIMP-2 KO mice of both sexes were hyperglycemic, hyperinsulinemic, and hyperleptinemic at the study termination. n = 4–8 per sex, genotype, and diet. *, P < 0.05; **, P ≤ 0.01; #, P ≤ 0.001; ##, P < 0.0001.