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. Author manuscript; available in PMC: 2012 Mar 23.
Published in final edited form as: J Am Chem Soc. 2011 Feb 28;133(11):4018–4029. doi: 10.1021/ja109679q

Figure 8.

Figure 8

(a-c) Negatively stained TEM images of Cg1, Cg13, and Cg13s fibril samples. (d) ThT fluorescence intensity at 490 nm for seeded D23N-Aβ1-40 fibrils after 1 hr (white bars), 5 min (shaded bars), and 10 min (black bars) of incubation under growth condition C (pH 7.4, 37° C, agitated). (e-g) 2D spectra of Cg1, Cg13, and Cg13s fibrils, prepared from D23N-Aβ1-40 with uniform 15N and 13C labeling of Val18, Phe20, Ala30, Ile32, Leu34, Met35 and Gly38. The Cg13s sample was collected by ultracentrifugation and lyophilized after only 60 min of fibril growth. In all 2D spectra, color-coded dashed lines indicate the positions of intra-residue chemical shift assignment paths from the Cg1 spectrum. Solid lines indicate selected signals from the Cg13 spectrum that obviously differ from those in the Cg1 spectrum.