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Fig. 6. Gyp7p enhances Gdi1p-mediated Ypt7p release. Fusion reactions (90 µl) containing 18 µg of BJ3505 vacuoles only were incubated with Gdi1p or with Gdi1p and Gyp7p at 27°C. At the indicated times, 30 µl aliquots were removed, centrifuged at 16 000 g for 5 min, and equal portions of the supernatant and vacuole pellet fractions were analyzed by immunoblotting for Ypt7p.
