FIGURE 4.
Effect of 19-Atriol on TSPO binding parameters. A and B, binding and cross-linking of [3H]promegestone to synthetic TAT-CRAC peptide in the presence of increasing concentrations (0.1–100 μm) of cholesterol (A) and 19-Atriol (B). A representative autoradiograph of three independent experiments is shown. C, quantification of [3H]promegestone cross-linking to synthetic TAT-CRAC peptide in the presence of increasing concentrations (0.1–100 μm) of cholesterol and 19-Atriol. The mean ± S.E. (error bars) of three independent experiments is presented. *, p < 0.05; **, p < 0.01 by one-way ANOVA. D, saturation isotherm of [3H]PK 11195 binding to isolated MA-10 mitochondria in the presence and absence of 10 μm 19-Atriol. The mean ± S.E. of three independent mitochondrial preparations and binding experiments is shown. E and F, effect of 19-Atriol on Bmax (E) and Kd (F) of PK 11195 binding to MA-10 mitochondria (from D). Results shown are the mean ± S.E. of three independent mitochondrial preparations and binding experiments.