Figure 1. Neurotrophin expression was examined in the geniculate ganglion, the anterior tongue and soft palate.
After dehydration, the geniculate ganglion (outlined by dotted line) was visualized in transverse tissue sections (A), and could be easily captured using laser capture microdissection (LCM). LCM successfully removed the ganglion but not the surrounding tissue (B). The anterior tongue, defined as the portion of the tongue immediately rostral to the intermolar eminence (C), was dissected and the epithelium and mesenchyme were separated for analysis. The soft palate was dissected by making a cut between the hard and soft palate, and then the tissue caudal to this cut was removed (D). The epithelium was separated from the connective tissue and developing glands underneath the epithelium for analysis. Scale bar in A equals 200 µm and applies to A and B.