TABLE 2.
Primer and probe sequences used in Q-RT-PCR assays
| Gene | Accession no. | Forward primer (+) and anti sense primer (−) | Probe (5′ FAMa) |
|---|---|---|---|
| gyrB | NC003997.3:4584-6506 | (+) ACTTGAAGGACTAGAAGCAG | CGAAAACGCCCTGGTATGTATA |
| (−) TCCTTTTCCACTTGTAGATC | |||
| atxA | NC001496.1:150042-151469 | (+) ATTTTTAGCCCTTGCAC | CTTTTATCTCTTGGAAATTCTATTACCACA |
| (−) AAGTTAATGTTTTATTGCTGTC | |||
| acpA | NC002146.1:68909-70360 | (+) ATTATCTTTACCTCAGAATCAG | CAATTTCTGAAGCCATTTCTAATCTT |
| (−) AACGTTAATGATTTCTTCAG | |||
| acpB | NC002146.1:49418-50866 | (+) TTTTTCAATACCTTGGAACT | CTTGAAGAATCATTAGGAATCTCATTACA |
| (−) AATGCCTTTTAGAAACCAC | |||
| capB | NC002146.1:56089-57483 | (+) TTTGATTACATGGTCTTCC | ATAATGCATCGCTTGCTTTAGC |
| (−) CCAAGAGCCTCTGCTAC | |||
| 16S rRNA | NC003997.3:9335-10841 | (+) TTCGGGAGCAGAGTG | CAGGTGGTGCATGGTTGTC |
| (−) AACATCTCACGACACGAG |
a
FAM, 6-carboxyfluorescein.