Fig. 2.
Experimental schemes to address the ISH in male GSCs. (A,B) Examples of BrdU staining in GSCs (encircled by dotted lines) from flies cultured in the absence (A) or presence (B) of BrdU for 24 hours. Red, Fas III and BrdU; blue, Vasa (germ cells); * indicates the hub. (C) Outcome of BrdU incorporation experiment with varying pulse periods. Data is shown as the frequency (%) of BrdU-positive GSCs/total GSCs (mean ± s.d.). 300–400 cells were scored for each data point. (D) The experimental scheme: newly eclosed flies were starved for 12 hours, followed by a 24-hour pulse period. They were then transferred to normal media for the indicated time. (E,F) Localization of Pav–GFP in male GSCs during telophase (E) and after mitosis (F). Red, Fas III; green, Pav–GFP; blue, Vasa (germ cells); light blue, DAPI; * indicates the hub; arrowheads point to Pav–GFP-marked contractile ring/midbody (A,C) or spindle midzone (B,D).