Figure 4. ADCYAP1R1 Methylation and mRNA expression.
A) Methylation within the first CpG island of ADCYAP1R1 (beta value, Illumina #cg27076139) is positively correlated with total PTSD symptoms (both sexes; N=107; r = 0.354, p < 0.0005). B) Subjects with PTSD have higher levels of ADCYAP1R1 methylation (median split, N = 107; chi-squared analyses, p < 0.005). C) ADCYAP1 mRNA levels are inversely correlated with ADCYAP1R1 mRNA levels in cortex (from prior dataset13)(r=−0.219; p<0.001). D) ADCYAP1R1 mRNA levels are differentially expressed in females compared to males based on imputed ADCYAP1R1 rs2267735 genotype (from prior dataset13)(*p<0.05 male vs. female CC carriers, # p<0.05, one-tailed, CC vs G-carriers). Bars represent mean ± SEM, N’s for each group at bottom of each graph.