Fig. 3. Western blots and glycoprotein stained synchronous sporulating culture samples.
Samples from synchronous cultures were taken, lysed, and resolved on 4–20% Tris-glycine gels. Identical immunoblots blotted with A) polyclonal anti-BxpB antibodies and B) polyclonal anti-BclA antibodies. Time points correspond to those of Fig. 1. The positions of a minor BclA complex (upper arrow) and monomeric BclA (lower arrow) are indicated. C) Glycoprotein stain of identical samples as A and B. Although a unknown glycoprotein complex is found at the same molecular weight as the initial BclA and BxpB complexes found in A and B, at T8 a smear of glycosylation is present ( >250 kDa) that corresponds to the increase in size of both BclA and BxpB complexes at that time point (asterisk).