Figure 3.
Phosphorylation of ATM and DNA-PKcs influences their dynamics at DNA double strand breaks. (A) AT5BIVA cells expressing wild-type, 1981A or kinase-dead (KD) forms of YFP-ATM were micro-irradiated, incubated for 10 min, pre-extracted, fixed and immunostained with a phospho-specific antibody to ATM (S1981). (B) 2 hour time course after micro-irradiation, showing the kinetics of YFP-tagged wild-type, 1981A and KD forms ATM in AT5BIVA cells. Each data point is the average of 10 independent measurements. Error bars represent the standard deviation. ©Sairei So et al. 2009; Originally published in J Cell Biol doi:10.1083/jcb.200906064 (C) 2 hour time course after micro-irradiation, showing the kinetics of YFP-tagged wild-type, 7A and KD forms DNA-PKcs in V3 cells. Each data point is the average of 10 independent measurements. Error bars represent the standard deviation. ©Naoya Uematsu et al. 2007; Originally published in J Cell Biol doi:10.1083/jcb.200608077.