Figure 1.
Accumulation and dissociation/retention kinetics of ATM and DNA-PKcs at laser-generated DNA double strand breaks. (A) Accumulation of YFP-tagged ATM and DNA-PKcs at laser-induced double strand breaks. Time-lapse imaging of exogenously expressed YFP-ATM and YFP-DNA-PKcs in HT1080 cells before and after micro-irradiation. Initial accumulation kinetics (180 seconds) of YFP-ATM and YFP-DNA-PKcs accumulating at the DSB site after micro-irradiation. (B) 2 hour time course after micro-irradiation, showing the kinetics of YFP-tagged ATM and DNA-PKcs in HT1080 cells. Each data point is the average of 10 independent measurements. Error bars represent the standard deviation.