Figure 7. Expression of Numb, Notch1-IC, and activated β-Catenin in early embryos.
Confocal images (maximum projection by z-stacking, XY planes) of whole mount immunostaining for (A) NUMB, (B) cleaved Notch1 intracellular domain (Notch1-IC) and (C) activated β-catenin in different staged mouse embryos and yolk sacs. Yellow arrows indicate the yolk sac; white arrow-heads indicate the posterior primitive streak region in neural plate stage embryos, and white arrows indicate the embryo proper. Dashed lines delineate the YS region in somite-stage embryos. Scale bars represent 50μm. Red: Numb, or Notch1-IC or activated β-catenin; Blue: DAPI