Figure 2.
(A) Structure of the 2-site ELISA for detection of antibodies against the cTfRMAb-ScFv fusion protein. The cTfRMAb-ScFv fusion protein is used as the capture reagent, and the biotinylated cTfRMAb-ScFv fusion protein is used as the detector reagent, along with a complex of streptavidin (SA) and horseradish peroxidase (HRP); the biotin moiety is designated, ‘B’. (B) Absorbance at 1:50 dilutions of AD mouse plasma taken at the end of the 12-week treatment study for mice in either the saline or the cTfRMAb-ScFv fusion protein treatment groups. The capture reagent in this experiment is the cTfRMAb-ScFv fusion protein. (C) Absorbance at 1:50, 1:300, 1:1000, and 1:3000 dilutions of a pool of terminal plasma from the 2 mice in the cTfRMAb-ScFv fusion protein treatment group that reacted the highest in the screen at 1:50 dilution (panel B). The capture reagent in this experiment is the cTfRMAb antibody, the rat 8D3 antibody, or mouse IgG1κ.