Fig. 7.
Effect of G-protein inhibition on ALR-induced synthesis of NO and cytokines in Kupffer cells. A: Kupffer cell membranes were incubated with the G-protein association mixture in the presence of 45 nM ALR without or with 50 ng/ml pertussis toxin (PX) or 2.5 μg/ml cholera toxin (CX) for 2 h, and membrane-associated radioactivity was determined. Specific [35S]GTPγS association (differences between binding in the absence and presence of excess unlabeled GTPγS) is shown. B–D: Cells were preincubated for 15 min in the absence or presence of 50 ng/ml pertussis toxin or 2.5 μg/ml cholera toxin, then stimulated with 45 nM ALR. After 24 h. NO, TNF-α, and IL-6 synthesis were measured. *P< 0.05 versus control; **P< 0.001 versus control; ***p< 0.01 versus control; #P< 0.05 and ##P< 0.001 versus ALR alone.