Figure 5.
Ethanol requires CRF and POMC to stimulate melanosome dispersal. A–G, Images of 5 dpf zebrafish larvae in light for 20 min (A–C) or upon treatment with 1.5% ethanol in light for 20 min (D–F). The control shows melanosome dispersal upon ethanol treatment (D) compared to no ethanol treatment (A). The pomc (E) or crf morphant (F) shows significant impairment of melanosome dispersal upon ethanol treatment; quantification (G) (***p < 0.001, n = 12, two-way ANOVA). H, I, Rescue of pomc (H) and crf morphant (I) phenotype with α-MSH treatment (***p < 0.001, n = 12, one-way ANOVA). J, Rescue of crf morphant phenotype with CRF peptide injection in the brain (***p < 0.001, n = 12, one-way ANOVA). K, Synergistic effect of pomc and crf morpholinos at suboptimal concentration on ethanol-mediated melanosome dispersal (***p < 0.001, n = 15, two-way ANOVA).