FIG. 6.
TLR2 but not TLR1 mRNA expression is upregulated in activated CD4+ T cells. CD4+ T cells (2 × 106) were either left unstimulated or stimulated overnight with anti-CD3 and anti-CD28 MAbs. Total cellular RNA was isolated, and TLR1 and TLR2 mRNA expression was measured by RT-PCR and normalized to β-actin mRNA levels. The TLR1 and TLR2 mRNA level in unstimulated CD4+ T cells was set at 1.0, and all other conditions were plotted as a relative measure to that value (relative comparative threshold value [CT]). Each bar represents the mean ± the SD of values from three experiments with different donors. *, P < 0.01 compared to no treatment control (unstimulated cells).