Fig. 4.
Subcellular distribution of NKCC1 and NBCe1 in villus enterocytes and goblet cells Villus sections of proximal jejunum were double labeled to detect NKCC1 (red), NBCe1 (green), and F-actin (red) using rhodamine-phalloidin. A: prominent membrane and intracellular NKCC1 staining in three goblet cells (G) among enterocytes (E). B: normalized NKCC1 fluorescence intensity (FI) at the lateral membrane and the intracellular apical pole in villus goblet cells vs. villus enterocytes. NKCC1 lateral membrane FI in enterocytes is 0.30 relative to goblet cells. Lower and upper villus cell data were combined; the individual cell group data are presented in Fig. 11E. Data shown are expressed as means ± SE. *P < 0.001; significantly different by Student's t-test. C: high-magnification image of a goblet cell (G) and neighboring enterocyte (E) double labeled for NBCe1 (green) and NKCC1 (red) and merged image. Intracellular NKCC1 in goblet cells (arrows) D: high-magnification views of double label for NBCe1/F-actin of duodenal villus enterocyte (E) and neighboring goblet cell (G) Side views (top) and en face views (bottom) show NBCe1 (green) colocalization (arrowheads) with F-Actin (red) of the enterocyte (E) but not the goblet (G) cell membrane (open arrowheads). Scale bars: A and C, 10 μm; D, 5 μm.