Table III.
Pre-treatment of B. burgdorferi-infected C3H mice with both anti-IFNAR1 and anti-IFNγ abolishes IFN-inducible transcript expression within joint tissue at 1 wk post-infection
| Pre-treatment of B. burgdorferi-infected mice | Transcript levels in joint tissue* |
|||
|---|---|---|---|---|
| Igtp | Iigp1 | Oasl2 | Cxcl9 | |
| PBS (5) | 14.0 ± 8.1 | 63.7 ± 11.3 | 5.1 ± 0.8 | 80.7 ± 23.5 |
| MOPC-21 + Hamster IgG (5) | 10.8 ± 7.9 | 55.1 ± 28.9 | 6.3 ± 4.2 | 87.2 ± 58.0 |
| Anti-IFNAR1 + Anti-IFNγ (5) | 0.6 ± 0.3† | 1.0 ± 0.4† | 0.4 ± 0.3† | 1.7 ± 0.8† |
| Uninfected (16) | 1.1 ± 0.4 | 3.0 ± 1.3 | 1.8 ± 0.9 | 5.3 ± 2.7 |
| % Reduced by α-IFNAR1 + α-IFNγ‡ | 100 | 100 | 100 | 100 |
*
Transcript levels determined by RT-PCR, normalized to 1000 copies of β-actin
†
Significantly different from both PBS and isotype controls, p < 0.05 by Mann-Whitney U test
‡
Average reduction seen versus PBS and IgG1 controls when compared with untreated, uninfected mice