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. 2010 Nov 10;85(2):753–764. doi: 10.1128/JVI.01835-10

TABLE 1.

Rotavirus shedding in stools of vaccinated mice after challenge with murine rotavirus (EDIM strain)a

Parameter affected by immunization (no. of mice)b Value at indicated no. of days after virus challenge
 Overall value
1 2 3 4 5 6
WT P particle (control) (7)
    Virus shedding
        Mean 692.8 4,215.2 5,639.9 4,474.2 3,045.7 64.4
        Standard deviation 869.9 2,715.9 2,932.7 2,063.1 2,072.1 30.1
P particle-VP8 from Wa (5)
    Virus shedding (μg/ml)
        Mean 801.4 4,205.1 4,198.7 3,768.2 1,673.1 23.8
        Standard deviation 254.1 1,644.0 1,500.6 1,205.0 758.3 6.8
    % reduction in shedding −11.2 0.2 25.6 15.8 45.1 63.0 23.1
    P value 0.903 0.993 0.341 0.511 0.192 0.015* 0.101
Free VP8 from EDIM (5)
    Virus shedding (μg/ml)
        Mean 143.9 949.1 1,227.0 809.8 211.8 29.6
        Standard deviation 151.8 988.6 1,328.2 311.5 57.2 29.3
    % reduction in shedding 79.2 77.5 78.2 81.8 93.1 54.0 77.3
    P value 0.201 0.029* 0.011* 0.003** 0.013* 0.073 0.019*
P particle-VP8 from EDIM (5)
    Virus shedding (μg/ml)
        Mean 5.4 575.3 662.4 875.6 139.0 9.2
        Standard deviation 12.0 1,095.1 648.4 334.5 187.9 11.9
    % reduction in shedding 99.2 86.3 84.2 80.4 95.4 85.7 88.5
    P value 0.048* 0.018* 0.004** 0.005** 0.011* 0.003** 0.018*
a

Mice were immunized intranasally as described in Materials and Methods. Two weeks after the last immunization, mice were challenged with 105 50% shedding doses of murine EDIM. Stools were collected from each mouse for 6 days after challenge and analyzed for the quantity of rotavirus antigen.

b

Antigen shedding is per mouse per day in each group. Reduction in shedding is presented as the percent reduction in shedding relative to the control group, either by day or over 6 days. P values were calculated by comparison to the control group vaccinated with the wild-type (WT) P particle. A single asterisk indicates a statistically significant difference (P < 0.05), while a double asterisk indicates a statistically very significant difference (P < 0.01).