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Protonophore-induced release of the mitochondrial pool of DCF. Cells loaded with 10 μM DCFH-DA were exposed to 2 mM H2O2 for 1 min to reveal DCF staining. Application of 100 μM carbonyl cyanide m-chlorophenylhydrazone (CCCP) rapidly released DCF staining. Experiment was repeated 5 times with similar results, and a representative myocyte is shown.
