Fig. 7.
Locations of glycines (blue, shaded) in diverse RLC sequences suggest that a hinge in helix D is a conserved feature of myosin RLC. See text. Alpha-helices (underlined) and residue numbers are indicated for Placo SmoA. Placo SmoA and human smooth-muscle RLCs, which include the phosphorylatable RxxS sequence (italicized), show identical sequences for the nearby nine-residue stretch in the N-terminal extension (lowercase red), despite being from such different organisms (see text). (Note N-terminal extensions prior to helix A are not aligned.) The databank identification numbers for the listed RLC sequences are: 1389849 (1. Placo SmoA), p13543 (2. Argo striated), Protein Data Bank ID code 3I5G (3. Squid), 18143650 (4. Physarum), P02609 (5. chicken skeletal), BAB88917.1 or GI:29568111 (6. human smooth), NP_000423.2 (7. human slow cardiac RLC2).