Figure 1. CXCR3 and CCR5 are upregulated in the genital tract during infection and necessary for clearance of C. trachomatis.
A) RNA was isolated from tissues of uninfected and infected mice. RT-PCR was used to quantify the expression of five chemokine receptors relative to a GAPDH control. Fold-induction was calculated (relative expression infected)/(relative expression uninfected). The data shown are representative of three independent experiments. Each bar represents the average fold-induction ± SD of two pools of five mice for each condition B) Genomic DNA was isolated from the genital tract of wild-type, CXCR3−/−, or CCR5−/− mice six days following infection. Quantitative PCR was used to calculate the levels of C. trachomatis 16s DNA relative to levels of host GAPDH. Shown is a box and whisker plot of two combined experiments each conducted with three mice per genotype. * = p<0.05