Skip to main content
. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: J Immunol. 2010 May 21;184(12):7082–7091. doi: 10.4049/jimmunol.1000438

FIGURE 3. Profile of IFN-γ and IL-2 cytokine-secreting human cells in mouse spleens at 5 weeks after infection.

FIGURE 3

FACS plots are shown from three infected and one uninfected animals. The number of cells producing cytokines in response to in vitro stimulation by HIV-1 gag and envelope peptide pools was determined by ICS, and the results were compared to non-stimulated cells. Splenocytes were enriched for human CD45+ cells using magnetic bead separation method and stimulated with peptide pools for 18 hr with Brefeldin A included during the last 6hr. CD8+ (blue) and CD4+ (red) T cells producing IFN-γ and IL-2 are shown, and the numbers represent percentages of cells positive for each cytokine. The hierarchy followed for gating was CD45→CD3→CD8 or CD4→ IFN-γ or IL-2. IFN-γ and IL-2 double positive cells shown are from respective gates indicated by arrows.