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. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: Nat Immunol. 2006 Feb 19;7(4):392–400. doi: 10.1038/ni1311

Fig. 5.

Fig. 5

MAZR-induced variegation in wild-type, E8I- and E8II-deficient thymocytes (a) Representative GFP expression profile of thymocytes transduced with MIG-R and MAZR. Gating areas for GFP− (−) and GFP+ (+) cells (thin line rectangles), and high (hi) GFP-expressing cells (thick line rectangles) are shown. Scatter plots show the percentage of CD3lo (top) and CD5int (bottom) cells within the CD4 SP subset of MIG-R and MAZR transduced wild-type (wt; left), E8I-deficient (Δ1/Δ1; middle) or E8II-deficient (Δ2/Δ2; right) thymocytes, respectively. Each circle represents one mouse. Horizontal bars indicate average value. (b) Left, representative histograms of GFP+ thymocytes indicating gating areas for CD4+CD8-CD3lo, CD4+CD8-CD3hi and DP thymocytes, respectively, that were used to determine expression as shown in the right panel. Right, expression of the zinc finger transcription factor Th-POK in MAZR-trasnduced thymocytes. Semi-quantitative RT-PCR of Zbtb7b (Th-POK) mRNA levels in CD4+CD3lo, DP, and CD4+CD3hi populations of MAZR-transduced E8I-deficient thymocytes. Open wedges indicate undiluted sample, 1:3, and 1:10 dilutions, respectively. M, marker; −, RT(−) control. Hprt1 mRNA was used as quantification control.