Fig. 1.
Directional release of RSV from polarized or nonpolarized cell culture monolayers. Polarized MDCK cell monolayers were infected with RSV from the apical (a) or basolateral surface (c). (b) Nonpolarized HEp-2 cell monolayers were infected with RSV from the apical surface. Apical (•) and basolateral (○) tissue culture supernatants were collected separately at the indicated times postinfection, and virus was quantified by plaque assay. The dashed line denotes the limit of detection for this assay. Titers at each time point are the average value of triplicate samples; the data are representative of at least two experiments.