Fig. 6.
Expression of ACBP and other key long-chain fatty acyl-CoA binding proteins in pre-implantation embryos. Embryos were isolated from ACBP heterozygote intercross mice, and levels of ACBP, sterol carrier protein-2 (SCP-2), sterol carrier protein-x (SCP-x), peroxisome proliferator-activated receptor-α (PPARα), and m-calpain were determined by Q-rtPCR as described in “Methods”. a Acbp, Scp-2, Scp-x, and Pparα expression was determined in homozygous ACBP-null embryos (open bars) and wild-type embryos (solid bars) at the 2.5-dpc morula stage (E3). Values represent means ± SEM; *p < 0.05 between ACBP-null and wild-type embryo; ***p < 0.0001 between ACBP-null and wild-type embryo. b Acbp and m-calpain expression was determined in homozygous null ACBP, heterozygous ACBP, and wild-type ACBP embryos at the morula (E3, 2.5 dpc) and early blastocyst (E4, 3.0 dpc) stages. (∞) indicates no live embryos found. (−/−) homozygous ACBP null; (−/+) ACBP heterozygous; (+/+) wild-type embryos