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. Author manuscript; available in PMC: 2011 Nov 1.

Published in final edited form as: Mol Immunol. 2010 Oct 16;48(1-3):73–81. doi: 10.1016/j.molimm.2010.09.009

DNA methylation patterns in naïve CD4⁺ T cells, in response to polyclonal T cell activation and in naïve CD4⁺ T cells, cultured under Th0 conditions in the presence of IL-27.

A) Methylation status for each CpG site. Within any given ROI, each circle represents a single clone sequenced for a specific CpG. The number of CpGs in each ROI are shown horizontally and the number of clones sequenced for each CpG is shown vertically. Closed circles depict a methylated and open circles identify unmethylated CpG sites. Methylation status for human IL10 (upper panel) and mouse Il10 (middle panel) in hIL10BAC mice and mouse Il10 (lower panel) in WT mice in naïve and activated T cells are shown. For selected ROIs, methylation of IL10 in naïve CD4⁺ T cells, cultured under Th0 conditions in the presence of IL-27 is shown.

B) Gene-wide methylation index (MI) of IL10 genes in WT and hIL10BAC mice. MI is defined as the percentage of methylated CpGs out of all CpGs over mouse and human IL10. Light grey bars indicate naïve and dark grey bars indicate activated CD4⁺ T cells.

C) Comparison of mouse (top-diamonds) and human (bottom-triangles) IL10 methylation in naïve (dashed line) and polyclonally activated (solid line) CD4⁺ T cells of hIL10BAC mice. The top and bottom portions are mirror images of one another with 0 in the middle being no methylation on each scale and 100 being equivalent to 100% methylation.

D) Comparison of naïve (top) and polyclonally activated (bottom) methylation patterns of mouse Il10 in WT (circles) and hIL10BAC mice (diamonds), and human IL10 (triangles) in hIL10BAC mice.

E) Comparison of mouse (top) and human (bottom) IL10 MI in naïve (diamonds), polyclonally activated (circles), and Th0 cells cultured with IL-27 (squares) in IL10BAC mice.

DNA methylation patterns in naïve CD4⁺ T cells, in response to polyclonal T cell activation and in naïve CD4⁺ T cells, cultured under Th0 conditions in the presence of IL-27.

A) Methylation status for each CpG site. Within any given ROI, each circle represents a single clone sequenced for a specific CpG. The number of CpGs in each ROI are shown horizontally and the number of clones sequenced for each CpG is shown vertically. Closed circles depict a methylated and open circles identify unmethylated CpG sites. Methylation status for human IL10 (upper panel) and mouse Il10 (middle panel) in hIL10BAC mice and mouse Il10 (lower panel) in WT mice in naïve and activated T cells are shown. For selected ROIs, methylation of IL10 in naïve CD4⁺ T cells, cultured under Th0 conditions in the presence of IL-27 is shown.

B) Gene-wide methylation index (MI) of IL10 genes in WT and hIL10BAC mice. MI is defined as the percentage of methylated CpGs out of all CpGs over mouse and human IL10. Light grey bars indicate naïve and dark grey bars indicate activated CD4⁺ T cells.

C) Comparison of mouse (top-diamonds) and human (bottom-triangles) IL10 methylation in naïve (dashed line) and polyclonally activated (solid line) CD4⁺ T cells of hIL10BAC mice. The top and bottom portions are mirror images of one another with 0 in the middle being no methylation on each scale and 100 being equivalent to 100% methylation.

D) Comparison of naïve (top) and polyclonally activated (bottom) methylation patterns of mouse Il10 in WT (circles) and hIL10BAC mice (diamonds), and human IL10 (triangles) in hIL10BAC mice.

E) Comparison of mouse (top) and human (bottom) IL10 MI in naïve (diamonds), polyclonally activated (circles), and Th0 cells cultured with IL-27 (squares) in IL10BAC mice.