Fig. 4.
The synaptic proteins GluR1 and Homer are up-regulated in the NAc of rodents with a history of excessive alcohol drinking. (A) Rats experienced the same procedure as Fig. 3C (n = 12–13 per group); *P < 0.05 and **P < 0.01, two-tailed unpaired t test. (B) C57BL/6J mice experienced the same procedure as Fig. 3A (n = 10 per group). Two-way ANOVA detected an interaction between the fluid (water or alcohol) and the treatment (Veh or Rapa) [F(1,36) = 4.34, P < 0.05]; *P < 0.05 and **P < 0.01 by Newman-Keuls post hoc test. The levels of GluR1 and Homer proteins were determined by Western blot analyses using pan antibodies. Data are presented as mean ± SEM and expressed as percentage of control.