Figure 3.
Primary cilia appear in airway epithelial cells before motile ciliogenesis in vitro and in developing lungs. (A) Preparations of mTEC were analyzed on indicated day of ALI culture by immunostaining, using antibodies for acetylated α-tubulin (α-tub, green) and γ-tubulin (γ-tub, red). Cells with primary cilia on ALI Day 1 decreased in abundance as motile cilia appeared on ALI Day 4, and as cells became well-differentiated on ALI Day 14. (B) Mouse lung sections from embryonic day (E) 12.5 were immunostained with antibodies to α-tub (green) and Arl13b (red), and show primary cilia. (C) Transition from primary to motile cilia during development. Lungs on E14.5 and E15.5 were immunostained for primary and motile cilia protein Arl13b (green) and motile cilia protein β-tubulin–IV (β-tub-IV, red). Insets show detail. Primary cilia (arrows) and motile cilia (arrowheads) are indicated. All bars = 10 μm.