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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biomaterials. 2010 Oct 20;32(3):926–932. doi: 10.1016/j.biomaterials.2010.09.058

Figure 4.

Figure 4

Chitosan/OVA/IL-12 vaccine induces production of CD8+ T cells that recognize MHC class I-restricted antigen peptide. Mice (n = 5) were vaccinated on days 0 and 14 with OVA ± chitosan + IL-12 at doses of 0.25, 1, or 4 μg IL-12, and splenocytes were harvested on day 21. B cell-depleted splenocytes were pooled and stained with FITC-CD8 and either PE-labeled OVA257-264 (SIINFEKL) or PE-labeled HSV498-505 (SSIEFARL) MHC class I pentamer. Data are expressed as the percentage of CD8+ cells that were positive for MHC class I pentamer. Results are representative of 2 independent experiments with similar trends.