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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: Cell Stem Cell. 2008 Mar 6;2(3):274–283. doi: 10.1016/j.stem.2008.01.003

Figure 2. Assessment of canonical Wnt signal activity in HSC-containing populations of Dkk1-tg mice.

Figure 2

Dkk1-tg mice were crossbred with Topgal transgenic mice. Three pairs of topgal+ littermates, with and without the Dkk1-tg were used to isolate the HSC-containing LK+S+ primitive fraction, and more differentiated LK+S− cells. FACS-sorted populations were used to isolate mRNA, and assessed for expression of the Topgal reporter. Data are expressed as the relative Topgal mRNA expression, compared to the levels present in LK+S+ wt cells (A). Three pairs of Dkk1-tg and wt littermates were also used isolate the LK+S+ fraction of BM, from which mRNA was isolated (non-Topgal transgenics). mRNA was also isolated from the tibea (bone + marrow) of wt and Dkk1-tg mice. Quantitative PCR was performed using primers specific for the Dkk1 transgene. Data are expressed as the relative amount of Dkk1 mRNA compared to that found in wt tibea (B), and error bars indicate SE of the mean.