Table 1.
Affinity and kinetic parameters (with background subtracted)
| mAb ID | ka, 1/Ms | kd, 1/s | KA, 1/M | KD, M | RMax, RU |
|---|---|---|---|---|---|
| 5A8 | 3.6 × 105 | 2.0 × 10−4 | 2.1 × 109 | 1.5 × 10−9 | 172 |
| 3C7 | 1.7 × 105 | 7.6 × 10−4 | 5.2 × 108 | 5.6 × 10−9 | 133 |
| 3D6 | 1.8 × 106 | 1.2 × 10−4 | 1.5 × 1010 | 8.0 × 10−11 | 294 |
| 5D12 | 5.4 × 105 | 3.2 × 10−4 | 2.0 × 109 | 7.0 × 10−10 | 272 |
| 3G9 | 9.0 × 105 | 1.9 × 10−4 | 4.7 × 109 | 2.4 × 10−10 | 268 |
For each antibody, the figures shown are the mean of 2 separate series of experiments, using separately prepared sensor chips in each case, where: ka indicates kinetics of association; kd, kinetics of dissociation; KD, dissociation rate constant (measure of affinity); KA, association rate constant; and RMax, maximum SPR response signal.