FIG. 1.
In vitro RCs of recombinant NL4-3-derived viruses encoding patient-derived Gag-Protease sequences. The distribution of viral RC is shown for samples collected during acute/early infection (n = 66) (a) and chronic infection (n = 803) (b). Viruses were generated and RCs were measured by using a GFP reporter T-cell line, as described in Materials and Methods. The average RC was determined for each recombinant virus using data from two (acute/early infection) or three (chronic infection) independent assays. Results were normalized to mean RCs of WT NL4-3 controls assessed in parallel such that values greater than or less than 1.0 indicate viruses that replicated faster or slower than NL4-3, respectively. Gag-Protease RC values were not significantly different between cohorts (the median RC was 1.01 for both cohorts) and were distributed normally around means of 0.98 (±0.12) for acute/early infection samples and 1.00 (±0.15) for chronic infection samples.