FIG. 8.
Induction of EGR1, NAB2, EBV Rta, and EBV ZEBRA protein expression in Akata cells treated with anti-IgG is blocked by an inhibitor of protein kinase C. Akata cells were untreated (−) or treated (+) with anti-IgG. Aliquots of each group of cells were untreated (−) or treated (+) with bisindolylmaleimide X (Bis X), a PKC inhibitor. Cell extracts prepared at time zero (0) or at 1, 2, 3, or 4 h were analyzed by immunoblotting for expression of EGR1, NAB2, EBV Rta, EBV ZEBRA, or β-actin by immunoblotting with specific antibodies. Cell extracts from the same experiment were utilized for panels A to C. In panel A, the immunoblot was developed with 125I protein 4. Panels B and C represent separate gels that were developed with ECL.