Figure 7.
Functional relationships between p68 and cohesin. (A) GST pull-down assay from HeLa NEs (top panel) or in vitro translated SMC1 (bottom panel) using immobilized GST only or recombinant GST-p68. Bound proteins were analyzed by Western blot for the cohesin subunit SMC1; p72 served as a positive control for in vivo analysis. (B) ChIP-qPCR analysis of isolated DNA associated with the cohesin subunits SMC1 and SMC3 at the CTCF-binding site of IGF2/H19 ICR in p68-depleted HeLa cells. (C) ChIP-qPCR analysis of isolated DNA associated with SMC3 at the CTCF-binding site of H19 ICR in SRA-depleted HeLa cells. (CK) Control. (D) ChIP-qPCR analysis of isolated DNA associated with p68 at the CTCF-binding site of IGF2/H19 ICR in SA2-depleted HeLa cells.