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A) Protein expression was assessed in our panel of melanoma cell lines by western blot. Note expression of NEDD9 protein by cells that are metastatic in vivo (in blue). B) Western blot confirming GFP-NEDD9 overexpression (SK-MEL24, SK-MEL28 and SK-MEL187) or shRNA downregulation of NEDD9 protein (A375, A2058 and WM2664) compared to controls.
