Figure 7.
Morphology and tracer coupling pattern of an M2 ipRGC. (A) Confocal (negative) images showing a M2 cell (asterisk) filled with the loose-patch technique. This very well-filled M2 cell exhibited intense tracer-coupling to displaced amacrine cells. Two types of coupled amacrine cells can be seen (large cells arrows, smaller cells arrowheads). (B) High-power photomicrograph (inset from A) showing the detailed morphology of one of the coupled cells of panel A. Arrows show axon-like processes. (C) Drawings of two displaced amacrine cells coupled to the M2 cell in A. These amacrine cells have short dendrites and axon-like processes (arrows). Note that the staining of the amacrine cells drawn is incomplete due to limited tracer diffusion into coupled cells. Scale bars (A) = 50 μm, (B) = 12.5 μm, (C) = 100 μm.