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IpRGCs labeled by tdTomato in mouse retina. (A) Fluorescence of tdTomato-expressing ipRGCs within the GCL in living whole-mount retina. The tdTomato signal was strongest in the somata but, on occasion, (B) was detectable in the dendrites (arrows) and axons (arrowhead) of ipRGCs. (C) Fluorescence of a tdTomato-expressing ipRGC after fixation. (D) Melanopsin immunofluorescence (Alexa 488 secondary) demonstrating colocalization with tdTomato. Scale bar A, B: 40 μm; scale bar C, D: 10 μm.
