Figure 7. Pathology of murine infection with H37Rv, ΔctpV, and ΔctpV::ctpV.
A) H&E stained mouse lung tissue (40× magnification, scale bar = 500 μm) at 8 weeks post-infection. Inset images (1000× magnification, scale bar = 20 μm) show the Mtb bacilli (purple) which were visible in lung tissue starting from 8 weeks forward. B) H&E stained mouse lung tissue (40× magnification) at 38 weeks post-infection. C) Immunohistochemisty of infected murine lung tissue at 8 weeks post-infection. Murine lung tissue was sectioned and stained with antibody for mouse IFN-γ, which appears brown in the images (40× magnification). Inset images (200× magnification) of lesions displaying IFN- γ expression. D) Immunohistochemisty of infected murine lung tissue at 38 weeks post-infection. Inset images (200× magnification) of lesions displaying IFN- γ expression.