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. 2010 Nov 1;21(21):3680–3692. doi: 10.1091/mbc.E10-06-0527

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 4. — Bik1 promotes cMT growth in A. gossypii. (A) Anti-α-tubulin immunostainings in bik1Δ and wild-type cells. Pictures correspond to stacks of 5Z planes with 0.75-μm distance. (B) 1Z plane time-lapse microscopy of bik1Δ and wild-type cells expressing GFP-Tub1. Here, we focused on growth (arrows), pause (*), and shrinkage (arrowheads) of cMTs. (C) Quantification of cMT length in bik1Δ cells. The average WT cMT polymerization rate is shown as reference (black line). (D) Quantification of SPB position in bik1Δ and WT hyphae. SPBs are categorized into two classes: SPB situated in the white cortical zones and SPB situated in the center of the cell (gray zone). Bars, 5 μm.