Figure 3.
cMT interactions with the cell cortex generate SPB movements in A. gossypii cells. (A) Examples of 1Z plane time-lapse movies with GFP-Tub1 cells. a, cMT grows and sweeps the cortex. The SPB does not significantly move laterally but is pushed longitudinally. Yellow arrows represent the position of the SPB at t0. b, cMT grows and pushes the SPB against the cortex. c, cMT grows and slides along the cortex resulting in a longitudinal SPB movement. a–c, dashed lines represent the uppermost positions of SPBs in the image sequences. The cMT +ends are marked with white arrows. (B) A cMT grows and then slides along the cortex in a GFP-Tub1 Bik1-Cherry hypha (sliding occurs at t36–t54). Asterisk (*) shows a Bik1 dot moving along the cMT. Numbers indicate time in seconds. Bars, 5 μm.
