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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2010 Nov;34(11):1835–1842. doi: 10.1111/j.1530-0277.2010.01271.x

Fig.7. Effects of dbcAMP on the ability of ethanol-activated microglia conditioned media to induce apoptosis of MBH neurons.

Fig.7

Microglial cells were isolated and cultured in 24 well plates (1×105) and treated for 24 h with a 50 mM dose of ethanol without (E) or with a 1µM/ml dose of dbcAMP (E + cAMP), or with a 1µM/ml dose of dbcAMP alone (cAMP) for a period of 24 h. Control cultures were treated with vehicle only (C). Cell lysates were used to determine apoptotic death using a nucleosome ELISA. Each bar represents mean ± SEM of 5–6 samples. *P< 0.01, as compared to C. aP< 0.001, as compared to E-CM. bP< 0.001, as compared to E-CM+cAMP.